CliPE epegRNA Designer

# epegRNA designs

# VUS variants

# PLP variants

# BLB variants

epegRNA designs

Downloads

epegRNA distribution

Design strategy:

Introduce missense VUS variants

Introduce missense PLP/BLB variants

Gene Name

Transcript (Refseq)

Number of epegRNA libraries to design

Choose missense ClinVar tsv to upload

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Choose gnomAD csv to upload

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Include additional variants in editing windows:

Benign/Likely Benign variants

Pathogenic/Likely Pathogenic variants

gnomAD variants with allele count >= 5

PTC variants to induce LoF for assay validation/calibration

Additional Options

RTT Length

PBS Length

Disrupt PAM/Seed region? (synonymous variants)

Yes

No

gnomAD minimum allele count